Drug Resistance

Abstract

Despite significant progress in the treatment of HIV/AIDS, drug-resistant strains and central nervous system (CNS) complications remain a challenge. Astonishingly, a groundbreaking study reported three novel CNS-targeting HIV-1 protease inhibitors (PIs), offering hope for patients with drug-resistant HIV infection and HIV-associated CNS issues. The promising findings were recently published in Antimicrobial Agents and Chemotherapy.

Introduction

Since the discovery of Human Immunodeficiency Virus (HIV), the scientific community has been fighting an ongoing battle against the progression of the virus and its terminal stage, Acquired Immunodeficiency Syndrome (AIDS). Antiretroviral therapy (ART) has significantly reduced HIV-related morbidity and mortality rates. However, the emergence of drug-resistant viral strains and the limited efficacy of many ART drugs in treating HIV-related CNS complications render the need for novel therapeutics crucial.

Recently, a multi-institutional research team, spearheaded by Masayuki Amano from Kumamoto University, has uncovered a novel class of HIV-1 protease inhibitors that may hold the key to managing drug-resistant strains of HIV and counteracting the effects the virus has within the CNS. Their study, entitled “Novel Central Nervous System (CNS)-Targeting Protease Inhibitors for Drug-Resistant HIV Infection and HIV-Associated CNS Complications”, presents hopeful results for the future of HIV treatment.

CNS Complications and the HIV Reservoir Challenge

HIV-associated neurocognitive disorders (HAND) afflict a sizeable proportion of HIV-infected individuals, often resulting from the virus infiltrating the CNS. The blood-brain barrier (BBB), which serves as a safeguard for the brain against potential pathogens, also restricts many antiretroviral drugs from reaching the infected CNS cells.

Another challenge lies in the latent viral reservoirs, where HIV can lie dormant and undetected by the immune system, as well as by antiretroviral drugs. These reservoirs, especially those within the CNS, present significant obstacles to curing HIV, as the virus can re-emerge from these sanctuaries and reignite the infection.

Innovative Protease Inhibitors Targeting the CNS

The novel protease inhibitors reported in the study, GRL-083-13, GRL-084-13, and GRL-087-13, were engineered with CNS targeting in mind. They incorporated a P1-3,5-bis tetrahydropyrone (Tp) moiety, aiming to enhance permeability across the BBB and inhibitory potency against drug-resistant viral strains.

In vitro studies and preliminary animal model investigations demonstrated these inhibitors’ exceptional ability to inhibit HIV-1 strains that had developed resistance to current PIs. This remarkable potency against such strains is critical in addressing the growing issue of antiretroviral resistance.

Additionally, the innovatively designed CNS-targeting PIs showed promise in crossing the restrictive BBB, a property that could revolutionize HIV treatment by effectively targeting the CNS-based HIV reservoir.

Scientific Collaboration and Funding

The research was a collaboration between Kumamoto University, the National Cancer Institute, Purdue University, and others. It received financial backing from NIH grants (R01 GM053386, R37 AI150466, and R37 GM053386), highlighting the importance of continued investment in HIV/AIDS research.

Challenges Ahead

While the findings represent a significant stride forward, developing drugs that can effortlessly cross the BBB while retaining antiviral potency remains a challenge. Additionally, understanding how these PIs interact with other drugs and how they could be integrated into current ART regimens is crucial.

Conclusion

By introducing a set of effective PIs capable of breaching the BBB barrier and combating resistant HIV strains, this study offers a beacon of hope to HIV-infected individuals experiencing CNS complications. Further clinical development and testing are needed, but the potential impact on the lives of those affected by drug-resistant HIV could be revolutionary.

References

1. Amano, M., et al. (2019). Novel Central Nervous System (CNS)-Targeting Protease Inhibitors for Drug-Resistant HIV Infection and HIV-Associated CNS Complications. Antimicrob Agents Chemother, 63(7). doi:10.1128/AAC.00466-19.
2. Lohse, N., et al. (2007). Improved survival in HIV-infected persons: consequences and perspectives. J Antimicrob Chemother, 60, 461–463. doi:10.1093/jac/dkm241.
3. Mitsuya, H., et al. (2008). Development of protease inhibitors and the fight with drug-resistant HIV-1 variants. Adv Pharmacol, 56, 169–197. doi:10.1016/S1054-3589(07)56006-0.
4. Ghosh, A.K., et al. (1998). Potent HIV protease inhibitors incorporating high-affinity P2-ligands. Bioorg Med Chem Lett, 8, 687–690. doi:10.1016/S0960-894X(98)00098-5.
5. Amano, M., et al. (2007). A novel bis-tetrahydrofuranylurethane-containing nonpeptidic protease inhibitor (PI), GRL-98065, is potent against multi-PI-resistant human immunodeficiency virus in vitro. Antimicrob Agents Chemother, 51, 2143–2155. doi:10.1128/AAC.01413-06.

Keywords

1. HIV Protease Inhibitors
2. CNS-Targeting Antiretrovirals
3. Drug-Resistant HIV Treatment
4. HIV-Associated Neurocognitive Disorders
5. Blood-Brain Barrier Penetrating Drugs